We are in the midst of a new genomic era, where high-throughput sequencing approaches and genome engineering are revolutionizing research in the biological sciences. These technological advancements promise to revolutionalize our understanding of gene regulation.
Enabled by transcriptome sequencing, novel species of RNA have recently been discovered to be abundantly expressed in the nervous systems of animals ranging from fruit flies to humans. These include extended 3'UTR mRNA isoforms (Smibert, Miura et al., 2012; Miura, Shenker et al., 2013) and circular RNAs (Westholm, Miura et al., 2014; Gruner et al., 2016).
The mission of the laboratory will be to decipher how these newly discovered RNA molecules are regulated and what are their physiological roles in cells. In particular, we are interested in how these RNAs might play roles in neurological disease.
Alternative Cleavage and Polyadenylation (APA)
A little primer on how deep sequencing is used to annotate new extended 3'UTR isoforms
What happens when a polyA factor is reduced in a fly's neurons.